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KMID : 0376419850090030287
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Chonbuk University Medical Journal
1985 Volume.9 No. 3 p.287 ~ p.296
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Endogenous Peroxidase in Hyperplastic Kupffer Cells induced by Methylcellulose
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Abstract
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To investigate the origin of Kupffer cells, Kupffer cell hyperplasia was induced by intraperitoneal injection of methylcellulese 3 times weekly for 1, 2, 3, and 4 weeks respectively. Then histological (H&E stain), histochemical (PAS and met_hylgreen pyronin stains), and enzyme histochemical(nonspecific esterase and SDH stain) examinations, and endogenous peroxidase stain of hepatic tissue fixed with perfusion of glutaraldehyde were performed.
The results were as follows.
1 . Hyperplasia of Kupffer cells with nodular aggregation of inflammatory cells in hepatic lobules are induced by methylcellulose treatment. These hyperplastic reaction and mitotic figures of Kupffer cells are more prominent in group with longer duration of treatment.
2. Hyperplastic Kupffer cells showed positive reaction for PAS stain comparison to Kupffer cells of control group. These positive reactions were due to phagocytized methylcellulose.
3 . Hyperplastic Kupffer cells slowed negative reactions to MGP and SDH stains.
4 . Hyperplastic Kupffer cells revealed strong activity to endogenous peroxidase, a marker enzyme of fixed phagocytic system, but the activity of nonspecific esterase, a marker enzyme of bone marrow-derived monocyte, was not recognized.
As the above results, Kupffer cells are proliferated by mitosis in the hepatic sinusoid, and the activities of endogenous peroxidase are strong, but the activity of nonspecific esterase are not recognized. So the origin of Kupffer cells might be local mesenchymal cells.
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